pathogenic strain e faecium atcc 700221 Search Results


van a strain  (ATCC)
98
ATCC van a strain
Van A Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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e faecium  (ATCC)
95
ATCC e faecium
Fig. 1 Phylogenetic tree constructed based on analysis of single-nucleotide polymorphisms (SNPs) of the core genes of 48 entercocci genomes, including the 21 isolates obtained from bovine feces in the present study. Entercoccus faecalis, Entercoccus <t>faecium,</t> Enterococcus hirae, Entercoccus durans, Entercoccus casseliflavus and Entercoccus gallinarum were compared using E. faecium strain <t>T110</t> as a reference
E Faecium, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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enterococcus faecium atcc 700221  (ATCC)
97
ATCC enterococcus faecium atcc 700221
Fig. 1 Phylogenetic tree constructed based on analysis of single-nucleotide polymorphisms (SNPs) of the core genes of 48 entercocci genomes, including the 21 isolates obtained from bovine feces in the present study. Entercoccus faecalis, Entercoccus <t>faecium,</t> Enterococcus hirae, Entercoccus durans, Entercoccus casseliflavus and Entercoccus gallinarum were compared using E. faecium strain <t>T110</t> as a reference
Enterococcus Faecium Atcc 700221, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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enterococcus faecalis atcc 29212 vancomycin sensitive enterococcus faecium atcc 700221 vre  (ATCC)
99
ATCC enterococcus faecalis atcc 29212 vancomycin sensitive enterococcus faecium atcc 700221 vre
Fig. 1 Phylogenetic tree constructed based on analysis of single-nucleotide polymorphisms (SNPs) of the core genes of 48 entercocci genomes, including the 21 isolates obtained from bovine feces in the present study. Entercoccus faecalis, Entercoccus <t>faecium,</t> Enterococcus hirae, Entercoccus durans, Entercoccus casseliflavus and Entercoccus gallinarum were compared using E. faecium strain <t>T110</t> as a reference
Enterococcus Faecalis Atcc 29212 Vancomycin Sensitive Enterococcus Faecium Atcc 700221 Vre, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nisin variants  (ATCC)
96
ATCC nisin variants
Determination of purity, yield and cleavage efficiency of the <t>pre-nisin</t> <t>variants.</t> (A) Purity of the purified nisin A and the variants nisin H and nisin H F 1 I (Marker: Precision Plus Protein Dual Xtra standards Bio-Rad). (B) Yields after purification of nisin A and their corresponding variants via cation-exchange chromatography. (C) Quantification of the cleavage efficiency of NisP. (D) Chromatogram of nisin H. (E) Chromatogram of the nisin H F 1 I variant. The pre-nisin variants before NisP cleavage were shown by blue dashed lines and after NisP cleavage by black lines. Error bars represent the standard deviation of at least three biological replicates.
Nisin Variants, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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strains  (ATCC)
99
ATCC strains
Determination of purity, yield and cleavage efficiency of the <t>pre-nisin</t> <t>variants.</t> (A) Purity of the purified nisin A and the variants nisin H and nisin H F 1 I (Marker: Precision Plus Protein Dual Xtra standards Bio-Rad). (B) Yields after purification of nisin A and their corresponding variants via cation-exchange chromatography. (C) Quantification of the cleavage efficiency of NisP. (D) Chromatogram of nisin H. (E) Chromatogram of the nisin H F 1 I variant. The pre-nisin variants before NisP cleavage were shown by blue dashed lines and after NisP cleavage by black lines. Error bars represent the standard deviation of at least three biological replicates.
Strains, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pathogenic strains  (ATCC)
99
ATCC pathogenic strains
Determination of purity, yield and cleavage efficiency of the <t>pre-nisin</t> <t>variants.</t> (A) Purity of the purified nisin A and the variants nisin H and nisin H F 1 I (Marker: Precision Plus Protein Dual Xtra standards Bio-Rad). (B) Yields after purification of nisin A and their corresponding variants via cation-exchange chromatography. (C) Quantification of the cleavage efficiency of NisP. (D) Chromatogram of nisin H. (E) Chromatogram of the nisin H F 1 I variant. The pre-nisin variants before NisP cleavage were shown by blue dashed lines and after NisP cleavage by black lines. Error bars represent the standard deviation of at least three biological replicates.
Pathogenic Strains, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vancomycin-resistant enterococcus faecium (vre) 700221  (Microbiologics Inc)
90
Microbiologics Inc vancomycin-resistant enterococcus faecium (vre) 700221
Determination of purity, yield and cleavage efficiency of the <t>pre-nisin</t> <t>variants.</t> (A) Purity of the purified nisin A and the variants nisin H and nisin H F 1 I (Marker: Precision Plus Protein Dual Xtra standards Bio-Rad). (B) Yields after purification of nisin A and their corresponding variants via cation-exchange chromatography. (C) Quantification of the cleavage efficiency of NisP. (D) Chromatogram of nisin H. (E) Chromatogram of the nisin H F 1 I variant. The pre-nisin variants before NisP cleavage were shown by blue dashed lines and after NisP cleavage by black lines. Error bars represent the standard deviation of at least three biological replicates.
Vancomycin Resistant Enterococcus Faecium (Vre) 700221, supplied by Microbiologics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Fig. 1 Phylogenetic tree constructed based on analysis of single-nucleotide polymorphisms (SNPs) of the core genes of 48 entercocci genomes, including the 21 isolates obtained from bovine feces in the present study. Entercoccus faecalis, Entercoccus faecium, Enterococcus hirae, Entercoccus durans, Entercoccus casseliflavus and Entercoccus gallinarum were compared using E. faecium strain T110 as a reference

Journal: BMC microbiology

Article Title: Comparative genomics of Enterococcus spp. isolated from bovine feces.

doi: 10.1186/s12866-017-0962-1

Figure Lengend Snippet: Fig. 1 Phylogenetic tree constructed based on analysis of single-nucleotide polymorphisms (SNPs) of the core genes of 48 entercocci genomes, including the 21 isolates obtained from bovine feces in the present study. Entercoccus faecalis, Entercoccus faecium, Enterococcus hirae, Entercoccus durans, Entercoccus casseliflavus and Entercoccus gallinarum were compared using E. faecium strain T110 as a reference

Article Snippet: The 27 compete genomes from NCBI included: E. hirae (2 strains; ATCC 9790, R17), E. faecium (13 strains; Aus0004, Aus0085, T110, 6E6, VRE001, E1, E745, E39, UW8175, NRRL B2354, ATCC 700221, EFE10021), E. faecalis (9 strains; LD33, L12, KB1, 62, D32, V583, DENG1, OG1RF, ATCC 29212), E. durans (1 strain; KLDS6_0933), E. gallinarum (1 strain; FDAARGOS163), and E. casseliflavus (1 strain; EC20).

Techniques: Construct

Fig. 3 a Phylogenetic tree of Entercoccus faecium genome sequences from the present study and complete genome sequences from the NCBI database based on analysis of single-nucleotide varients (SNVs) of the core genes. b Relatedness tree of E. faecium genome sequences from present study and complete genome sequences from the NCBI database based on Pearson correlation similarity matrix analysis of accessory genes. Origin of isolates are as indicated in the figures

Journal: BMC microbiology

Article Title: Comparative genomics of Enterococcus spp. isolated from bovine feces.

doi: 10.1186/s12866-017-0962-1

Figure Lengend Snippet: Fig. 3 a Phylogenetic tree of Entercoccus faecium genome sequences from the present study and complete genome sequences from the NCBI database based on analysis of single-nucleotide varients (SNVs) of the core genes. b Relatedness tree of E. faecium genome sequences from present study and complete genome sequences from the NCBI database based on Pearson correlation similarity matrix analysis of accessory genes. Origin of isolates are as indicated in the figures

Article Snippet: The 27 compete genomes from NCBI included: E. hirae (2 strains; ATCC 9790, R17), E. faecium (13 strains; Aus0004, Aus0085, T110, 6E6, VRE001, E1, E745, E39, UW8175, NRRL B2354, ATCC 700221, EFE10021), E. faecalis (9 strains; LD33, L12, KB1, 62, D32, V583, DENG1, OG1RF, ATCC 29212), E. durans (1 strain; KLDS6_0933), E. gallinarum (1 strain; FDAARGOS163), and E. casseliflavus (1 strain; EC20).

Techniques:

Fig. 4 a Blast atlas of 10 Enterococcus hirae strains isolated from bovine feces and E. hirae strain R17 mapped against E. hirae ATCC9790. b Blast atlas of the genomes of 3 Entercoccus faecium isolates from bovine feces and 12 complete E. faecium genomes from the NCBI database mapped against reference sequence E. faecium DO. Blast atlases were generated by GView Java package software [28] using both alignment length and percent identity cut-off values of 80%. Based on the reference genomes, phage and transposon related regions/loci are indicated on the altas diagram

Journal: BMC microbiology

Article Title: Comparative genomics of Enterococcus spp. isolated from bovine feces.

doi: 10.1186/s12866-017-0962-1

Figure Lengend Snippet: Fig. 4 a Blast atlas of 10 Enterococcus hirae strains isolated from bovine feces and E. hirae strain R17 mapped against E. hirae ATCC9790. b Blast atlas of the genomes of 3 Entercoccus faecium isolates from bovine feces and 12 complete E. faecium genomes from the NCBI database mapped against reference sequence E. faecium DO. Blast atlases were generated by GView Java package software [28] using both alignment length and percent identity cut-off values of 80%. Based on the reference genomes, phage and transposon related regions/loci are indicated on the altas diagram

Article Snippet: The 27 compete genomes from NCBI included: E. hirae (2 strains; ATCC 9790, R17), E. faecium (13 strains; Aus0004, Aus0085, T110, 6E6, VRE001, E1, E745, E39, UW8175, NRRL B2354, ATCC 700221, EFE10021), E. faecalis (9 strains; LD33, L12, KB1, 62, D32, V583, DENG1, OG1RF, ATCC 29212), E. durans (1 strain; KLDS6_0933), E. gallinarum (1 strain; FDAARGOS163), and E. casseliflavus (1 strain; EC20).

Techniques: Isolation, Sequencing, Generated, Software

Determination of purity, yield and cleavage efficiency of the pre-nisin variants. (A) Purity of the purified nisin A and the variants nisin H and nisin H F 1 I (Marker: Precision Plus Protein Dual Xtra standards Bio-Rad). (B) Yields after purification of nisin A and their corresponding variants via cation-exchange chromatography. (C) Quantification of the cleavage efficiency of NisP. (D) Chromatogram of nisin H. (E) Chromatogram of the nisin H F 1 I variant. The pre-nisin variants before NisP cleavage were shown by blue dashed lines and after NisP cleavage by black lines. Error bars represent the standard deviation of at least three biological replicates.

Journal: Frontiers in Microbiology

Article Title: Insights in the Antimicrobial Potential of the Natural Nisin Variant Nisin H

doi: 10.3389/fmicb.2020.573614

Figure Lengend Snippet: Determination of purity, yield and cleavage efficiency of the pre-nisin variants. (A) Purity of the purified nisin A and the variants nisin H and nisin H F 1 I (Marker: Precision Plus Protein Dual Xtra standards Bio-Rad). (B) Yields after purification of nisin A and their corresponding variants via cation-exchange chromatography. (C) Quantification of the cleavage efficiency of NisP. (D) Chromatogram of nisin H. (E) Chromatogram of the nisin H F 1 I variant. The pre-nisin variants before NisP cleavage were shown by blue dashed lines and after NisP cleavage by black lines. Error bars represent the standard deviation of at least three biological replicates.

Article Snippet: All bacteria used for minimum inhibitory concentration (MIC) determination of nisin variants [ Bacillus subtilis 168; S. aureus : MSSA strain ATCC 29213, MRSA/VISA strain ATCC 700699; E. faecium : ATCC 35667, ATCC 700221 (vancomycin resistant); E. faecalis : ATCC 29212, ATCC 51299 (vancomycin resistant)] were cultivated in Mueller-Hinton broth (MHB) at 37°C and shaking at 150 rpm.

Techniques: Purification, Marker, Chromatography, Variant Assay, Standard Deviation

Nisin mediated pore formation, visualized with the SYTOX green assay. The NZ9000Cm strain incubated with the SYTOX dye. After a stable baseline (∼200 s), one of the nisin variants (100 nM) was added (indicated with an arrow). The fluorescence signal was measured using a fluorolog (Horiba III). The rapid increase in fluorescence indicates pore formation. The black line represents the addition of nisin A, the red line nisin H, and the blue line nisin H F 1 I. As a control we added buffer shown as green line.

Journal: Frontiers in Microbiology

Article Title: Insights in the Antimicrobial Potential of the Natural Nisin Variant Nisin H

doi: 10.3389/fmicb.2020.573614

Figure Lengend Snippet: Nisin mediated pore formation, visualized with the SYTOX green assay. The NZ9000Cm strain incubated with the SYTOX dye. After a stable baseline (∼200 s), one of the nisin variants (100 nM) was added (indicated with an arrow). The fluorescence signal was measured using a fluorolog (Horiba III). The rapid increase in fluorescence indicates pore formation. The black line represents the addition of nisin A, the red line nisin H, and the blue line nisin H F 1 I. As a control we added buffer shown as green line.

Article Snippet: All bacteria used for minimum inhibitory concentration (MIC) determination of nisin variants [ Bacillus subtilis 168; S. aureus : MSSA strain ATCC 29213, MRSA/VISA strain ATCC 700699; E. faecium : ATCC 35667, ATCC 700221 (vancomycin resistant); E. faecalis : ATCC 29212, ATCC 51299 (vancomycin resistant)] were cultivated in Mueller-Hinton broth (MHB) at 37°C and shaking at 150 rpm.

Techniques: Incubation, Fluorescence, Control

MIC values for nisin A, nisin H, and nisin H F 1 I against different pathogenic strains.

Journal: Frontiers in Microbiology

Article Title: Insights in the Antimicrobial Potential of the Natural Nisin Variant Nisin H

doi: 10.3389/fmicb.2020.573614

Figure Lengend Snippet: MIC values for nisin A, nisin H, and nisin H F 1 I against different pathogenic strains.

Article Snippet: All bacteria used for minimum inhibitory concentration (MIC) determination of nisin variants [ Bacillus subtilis 168; S. aureus : MSSA strain ATCC 29213, MRSA/VISA strain ATCC 700699; E. faecium : ATCC 35667, ATCC 700221 (vancomycin resistant); E. faecalis : ATCC 29212, ATCC 51299 (vancomycin resistant)] were cultivated in Mueller-Hinton broth (MHB) at 37°C and shaking at 150 rpm.

Techniques: